-
The
composition of the vaccine
Vaccine
will have modified Bat - hybridoma cells that produce IgG1 and IgG3 that
attached to bat-FcyR1. Bat-FcyR1 receptor has hydrophobic region that can
associate with effector cells and trigger their activation.
-
The
specific target of your vaccine
Vaccine
will bound to Z1; as a result protect host infection from virus infection.
-
The
delivery method of vaccine
Hybridoma
must be injected intravenously.
-
Vaccine
production
·
Rabbit
or goat must be infected with weakened z1h1
·
Blast
cell from the host must be isolated.
·
Fuse
plasma cell with myeloma cell, to obtain hybridoma
·
Modify
batman hybrid Omaha so it will not trigger any immune response (it can be
obtained because all the antigens were hide in bat-stealth mode)
·
Grow
enough hybridoma For huge population
·
Finally,
purify IgG1 and IgG3
-
The
intended mechanism by which vaccine works
Bat-Hybridma
will produce huge amount of IgG1 and IgG3 which modified to have bat-FcyR1
attached to it. It is known that Z1N1 can engulf and use antibody for replication,
however it is not the case with Bat-antibodies. Bat-FcyR1 attracts different
immune system cells, such as Macrophages, Neutrophils, Eosinophils and Dendritic
cells. By using hydrophobic region it can consequently activate effector cell.
Moreover, bat-FcyR1 able to bind to usual FcyR1 receptor with higher affinity
than usual antibodies. In addition, antibodies
able to opsonize Z1N1 and prevent virus from the host penetration. Z1N1 virus can’t destroy bat-antibody,
because bat-FcyR1 attached to it.
-
Whether
your vaccine is prophylactic or therapeutic
Vaccine
can be used as therapeutic, because of the fast and effective response.
-
Potential
adverse reactions of vaccine
Hybridoma
will not be able to undergo class switching, to produce IgA and travel to the
mucous membrane. Consequently, mucous membrane of respiratory tract will have
no protection. It is better to use 1 vaccine with combination of the second
vaccine.
Activated macrophages can produce
cytokines, which may end up with cytokines storm. Consequently, it leads to ROS
production, which can lead to tissue damage.
The population that should receive your
vaccine
Vaccine can be effective for people who already
infected with virus, because it can trigger effective immune response from the
inside.
2.
Passive immunization:
– The
composition of vaccine
The second vaccine is passive immunity type vaccine. Again myeloma
cell and plasma cell (which produce IgA) will be used to produce hybridoma.
After that hybridoma will be modified to produce dimeric IgA with bat-FcαRI
receptor attached. Finally, antibodies will be purified.
– The
specific target(s) of your vaccine
Dimeric IgA will target Z1 (HA glycoprotein), because it is unique to the
zombie virus and will increase specificity. After attachment to the Z1, bat-FcαRI
receptor attached to the antibody will activate Monocytes, Macrophages,
Neutrophils and Eosinophils. Activation of these cells via bat-FcαRI is important,
since some viruses are able to use macrophages to travel down to lower
respiratory tracks [1]. Bat-FcαRI will help to neutralize virus.
– The
delivery method for your vaccine
Vaccine can be delivered to the person, by inhalator to the
respiratory ways.
– The
intended mechanism by which your vaccine works
Dimeric IgA will work as neutralizing antibody. It will
attach to the Z1 part of the virus and have resistance against virus, which
usually eat and reuse antibody, because of the bat-FcαRI. Attachment to the
virus, will protect host from the infection, because dimeric IgA will not allow
virus to enter to the cell in mucous membrane.
Bat - FcαRI receptor attached to the dimeric IgA. Bat - FcαRI
receptor able to bind to normal FcαRI receptor with higher affinity than normal
IgA. In addition, it has hydrophobic region that able to attach to the cell
membrane of Dendritic cell, Macrophages, Neutrophils and Eosinophils, in order
to induce response in them.
– Whether
your vaccine is prophylactic or
therapeutic
This vaccine is administered during the disease and
therefore therapeutic. It possible to use vaccine for prophylactic
purposes, because it gives protection
for the limited amount of time.
–
Your vaccination regimen
Vaccination must be done once a month, because antibody is
not produced in the host. After it time antibodies can be eliminated from the
body.
– Potential
adverse reactions from your vaccine
Activated macrophages can produce cytokines, which may end
up with cytokines storm. Consequently, it leads to ROS production, which can
lead to tissue damage.
– The
population that should receive your vaccine
Everyone should receive vaccine, because it is easily
transmitted through air.
Reference :
Mucosal Delivery of
Biopharmaceuticals.
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